(1) Bioresource Overview

Project description

The Brisbane Breast Bank (BBB) was initiated to support high quality clinical research projects in the breast cancer field. The bank operates from the University of Queensland Centre for Clinical Research (UQCCR), located alongside the Royal Brisbane and Women’s Hospital (RBWH) on the Metro North Health Services campus in Herston. The RBWH is the largest tertiary referral hospital in Queensland, providing specialist services to patients throughout the State (current population 4.84 million, approximately 20.3% of the Australian population, of which approximately 48.5% resides in the greater Brisbane area [1]). The RBWH also receives referrals from northern New South Wales, the Northern Territory and neighbouring countries in the South West Pacific.

The BBB aims to collect samples from every consenting breast surgery patient undergoing treatment at the RBWH. To this end, tumour tissue surplus to diagnostic requirements is snap frozen and banked, as well as being formalin-fixed and paraffin-embedded (FFPE) for longer-term storage and detailed histopathology annotation. This includes samples with benign, pre-invasive and invasive diagnoses. A blood sample is also taken at the time of surgery, and processed into various derivatives (serum, plasma, whole blood, buffy coat, Guthrie card). We also collect breast tissue from healthy donors undergoing reduction mammoplasty (cosmetic cases from The Wesley Hospital) and prophylactic mastectomy.

The BBB is unique in that it provides highly detailed curation of the banked tissue samples. Indeed, from each banked piece of tissue a section is cut and the histology reviewed by a pathologist. To this end, we can provide reliable information about a sample’s tumour cellularity, and true content of the specific piece of tissue to be supplied for research.

The BBB has supplied tumour samples to a host of targeted projects (e.g. [3, 4, 5, 6]), as well as a significant body of ongoing work. Importantly, BBB samples made up 10% of the total breast cancer samples studied by the International Cancer Genome Consortium (ICGC), resulting in several landmark studies ([2] and refs therein). Its growing normal breast tissue resource has been used in large consortia-based studies (e.g. [7]) as well as normal breast biology projects (e.g. [8]). The recent expansion to collection of metastatic deposits has underpinned ground-breaking work characterising the genome and transcriptome of brain metastases [9]. The BBB is currently conducting a longitudinal, prospective study (Circ.BR, Circulating Biomarkers of Relapse in Breast Cancer), involving the collection of serial blood samples, primary tumour tissue and a Quality-of-Life survey from selected RBWH patients at a high risk of disease recurrence or spread. Finally, it also operates as the Brisbane node of the National Breast Cancer Foundation (NBCF)-funded BROCADE (BReast Origin CAncer tissue DonatEd after death) rapid autopsy program.

Classification (1)

Human.

Species

Homo sapiens.

Classification (2)

biological samples and clinical data (where possible), including pathology, therapeutic intervention, disease recurrence and outcome.

Context

Spatial coverage

Description: Brisbane, Queensland, Australia.

Royal Brisbane and Women’s Hospital, Herston, Brisbane.

The Wesley Hospital, Auchenflower, Brisbane.

Northern boundary: –27/153

Southern boundary: –27/–152.99

Temporal coverage

The start date of the collection period for the bioresource was 2005, and the collection is ongoing.

Temporal coverage for accessibility

N/A

(2) Methods

Steps

  • BBB donors are recruited at the time of diagnosis of breast cancer, through the breast cancer clinic at the RBWH; or by surgeons in either the normal (reduction mammoplasty) or metastatic setting. Informed consent is required, and consent forms are managed and retained by the BBB according to standard operating procedures (SOP).
  • Hospital staff or BBB staff collect blood, which is processed and stored according to a SOP.
  • Tissue is couriered by BBB staff from surgical theatre to the pathology department, and where there is tissue surplus to diagnostic requirements, the BBB will collect and bank according to SOPs.
  • Patient information, clinical and pathology data is collected from pathology reports and medical records, and this data is stored alongside sample information in a secure SQL database (Caisis). Unique de-identifying codes are applied to the specimens; and are also recorded in the limited-access database. Data management and auditing occurs according to SOPs.
  • Experimental data is recorded for each sample, and thus integration of layers of information is possible, especially for those samples subjected to genomic analysis.
  • Samples are shipped by licensed providers in accordance with I.A.T.A., UN and CASA standards.
  • The BBB is integrated with other biobanks nationally through The Australian Breast Cancer Tissue Bank (ABCTB) ([10]).

Stabilization/preservation

Blood is collected in 2 × EDTA and 1 × clot activator tube.

Tissue is collected and couriered in sterile pots, then frozen in barcoded cryovials.

Type of long-term preservation

Formalin-Fixed Paraffin-Embedded (FFPE), frozen, viable frozen.

All frozen samples are barcoded and de-identified; and stored in temperature monitored freezers and liquid nitrogen dewars protected by swipe card access.

Storage temperature

Room Temperature (20°C): FFPE blocks; Guthrie cards.

–80°C: DNA, whole blood, and blood derivatives.

–196°C: tissue.

Shipping temperature from patient/source to preservation or research use

e.g. –170°C to –190°C (liquid nitrogen dry shipper; snap frozen tissue); –80°C (on dry ice; tissue); 0–4°C (on ice; tissue); room temperature (18–25°C; blood samples).

Shipping temperature from storage to research use

Dependent on tissue derivative; –56°C (on dry ice; frozen tissue); 0–4°C (on ice; blood, DNA); room temperature (18–25°C; FFPE block).

Quality assurance measures

Detailed pathology review performed on each banked piece of tissue, affording the BBB of level of histological curation unmatched by other local and international banks.

Standard methods of nucleic acid extraction, qualification and quantification, including by Qubit analysis and Bioanalyser, where appropriate.

The BBB’s standard operating procedures have been submitted to ISBER (International Society for Biological and Environmental Repositories), and to the Australasian Biospecimen Research Network. The BBB is certified through the NSW Biobanking certification program, which is based on the Canadian process developed by the Office of Biobank Education and Research (OBER), University of British Columbia (UBC) and the Canadian Tissue Repository Network (CTRNet).

Source of associated data

Associated data is manually collected from hospital medical records, AUSLAB (pathology information database), MOSAIQ (radiology oncology database), and CHARM (oncology database) and the Queensland Cancer Registry. Data is audited by cross-referencing, and regular manual checks.

Ethics Statement

The BBB operates fully within the remit of ethical approval from the Royal Brisbane and Women’s Hospital (RBWH; 2005/022 Molecular Pathology and Genetic Sub-Classification of Precancerous and Cancerous Breast Lesions) and The University of Queensland (UQ; 2005000785 Molecular Pathology of Breast Cancer). Additional site-specific ethics approval for the BROCADE autopsy study is covered under HREC/15/QRBW/547.

The patient consents to unspecified future research on their donated sample, allowing genetic analyses as well as more long-term projects such as xenografts.

Constraints

N/A

(3) Bioresource description

Object name

Breast Cancer.

Bioresource name

Brisbane Breast Bank.

Bioresource acronym or short name: BBB

Bioresource location

The University of Queensland Centre for Clinical Research (UQCCR), Building 71/918 Royal Brisbane and Women’s Hospital Herston, QLD 4029, AUSTRALIA.

Bioresource contact

Kaltin Ferguson k.ferguson2@uq.edu.au.

Identifier used

N/A

Bioresource type

Breast cancer.

Type of sampling

disease based.

Anatomical site

Breast.

Metastatic lesions.

Disease status of patients/source

Breast cancer.

Clinical characteristics of patients/source

Pre- and post-menopausal breast cancer patients.

Male breast cancer.

No exclusions on the basis of treatment regime.

Samples generally collected at first surgical resection, however recurrences and metastatic lesions are also banked.

Size of the bioresource

As at April 2017, there are 2167 consented donors.

Vital state of patients/source

Alive and post-mortem.

Clinical diagnosis of patients/source

Breast Cancer.

Pathology diagnosis

Normal breast, pre-invasive and invasive lesions are collected.

ICD50.

Control samples

Normal breast tissue harvested from reduction mammoplasty.

Distant cancer-associated normal tissue.

Blood.

Biospecimen type

Frozen tissue: 5 mm3 per cryovial, number dependent on tissue size; ~50% of donors;

FFPE archival blocks: number dependent on tissue size;

Blood: Whole blood, 1 vial (0.5 ml).
Serum, 3 cryovials; 1 ml/vial.
Plasma, 5 cryovials; 1 ml/vial.
Buffy coat, 2 cryovials; 1 ml/vial.
Guthrie (dried blood spot) card; 1 card with 5 spots.

Release date

2005.

Access criteria

Samples are made available following a presentation of the proposed project to the BBB operating committee (as per author list). The BBB operates without cost recovery, and as such each supported proposal is considered a collaborative research project. Restrictions include whether the bank can provide sufficient samples to ensure a statistically robust experiment can be executed; whether the proposed project is unique and likely to produce significant advances in knowledge or clinical practice, making best possible use of the samples; and when there is deemed to be no conflict of interest.

(4) Reuse potential

A non-exclusive approach is taken towards sample provision and thus samples from the same donor may be supplied for use in multiple projects. The collaborative approach taken to research agreements enhances the re-use potential of the banked samples, as detailed records for the various experiments per sample are kept and therefore extra data is able to be shared where appropriate. To date, the BBB has supplied materials to 14 internal and 15 external projects. Samples are provided at a proportion of 13% of banked tissue and 19% for blood, to both domestic and international researchers.